The Department of Foreign Affairs and Trade has confirmed two Australians were among the fatalities from a train crash in Spain last week.
Spanish authorities say they have identified 69 victims of the crash between two high-speed trains.
Support and guidance has been offered the Australians' families to assist with final care arrangements.
"The Department of Foreign Affairs and Trade is providing consular assistance to the families of two Australians who died in Spain," a DFAT spokesperson told SBS News.
"We send our deepest condolences to the families at this difficult time".
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Spanish authorities are under pressure to ascertain the cause of the crash after audio recordings of interactions between train drivers and a control centre were published by the media.
Investigators are focusing on the failure of the automatic train protection system and potential errors made by the rail control centre in Madrid.
"We are working day and night not only to support the victims' families, but also to establish every last detail of this tragedy and strengthen safety measures to ensure it never happens again," Spanish Prime Minister Pedro Sánchez Pérez-Castejón said.
Spain's transport minister, Oscar Puente, resigned on Tuesday after the crash. He also withdrew from the list of candidates for the general election due on November 29.
Following the collision last week, protesters including far-right groups gathered outside Spain's Ministry of Transportation in Madrid to demand the government's resignation.
Hundreds took to the streets to call for accountability, with the rail system thrust into political contention since January's crash.
Sánchez said his government would declare three days of national mourning and called for increased safety measures to restore confidence in the high-speed rail system.
— with additional reporting by the Associated Press.
